How to Measure Mitochondrial Performance
Measuring mitochondrial performance can reveal health outcomes before symptoms appear so that you can improve while it is easy. I’m posting this article to record my search results so I and other people can follow up confirming the information and make better discoveries.
Measure Mitochondrial performance
Mitochondrial performance can be measured using various assays that assess parameters like membrane potential, oxygen consumption, and enzyme activity. Common methods include fluorescence-based assays, high-resolution respirometry, and histochemical techniques to evaluate specific mitochondrial functions.
Methods to Measure Mitochondrial Performance
Common Techniques
| Technique | Description | Advantages | Drawbacks |
|---|---|---|---|
| High-Resolution Respirometry | Measures oxygen consumption rate (OCR) to assess mitochondrial function. | Direct measurement of function. | Requires specialized equipment. |
| Fluorescence-Based Assays | Uses fluorescent dyes to assess mitochondrial membrane potential and other parameters. | Real-time monitoring in live cells. | Some assays are not fixable. |
| Succinate Dehydrogenase Activity | Measures the activity of SDH, an enzyme in the mitochondrial membrane. | Provides insight into metabolic activity. | Requires specific conditions for accuracy. |
Specific Assays
| Assay Type | What It Measures | Fixable? |
|---|---|---|
| JC-1 Dye | Mitochondrial membrane potential | Some end-point assays are fixable. |
| Superoxide Detection | Mitochondrial superoxide production | Not fixable |
| Calcium Detection | Mitochondrial calcium levels | Not fixable |
| Mitochondrial Permeability Transition | Changes in mitochondrial permeability | Not fixable |
Considerations
- Cell Type: Different cell types may respond differently to assays, so it’s important to choose the right method based on the specific cells being studied.
- Dynamic vs. End-Point Assays: Dynamic assays provide real-time data, while end-point assays measure at a specific time, which may not capture transient changes.
These methods and assays provide valuable insights into mitochondrial health and function, essential for understanding various cellular processes.
Post Lyme Disease Syndrome
Antibiotics can cure disease but cause another. The treatment for Lyme Disease is doxycycline or amoxicillin.
People get sick after supposedly getting the cure for lyme disease but many suffer long term problems. If the antibiotic treatment cured the original disease, the antibiotics might cause the post treatment syndrome.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527730
A potential cure for Post Lyme Disease Syndrome could be restoring the biome. Think of it like surgeons treating an injury. The surgeon cut the patient to fix damage and then stitch up the wound after surgery. The same concept could be applied to drugs. Restoring the biome is the stitches.
Round Square Combinations are Surprising
All four of these solids are Round Squares. I thought I had a general rule for calculating the combinations when I found the first two but then I found another and another. Is there a general formula for all the possible combinations. What are the parameters? Numerically there could be 6 but I do not think geometrically 6 are possible. This gets more confusing for Round Triangles or Square Triangles.
Cylinder is the obvious round square.
Cones are round triangle. Two triangles for a square. Two cones form a round square.
Combinations of 3D shapes are either unions or intersections. The intersection of a cylinder and a wedge makes a Round Square Triangle. The union of two half height Round Square Triangles form a Round Square.
Intersection of two cylinders rotates 90 degrees from each other leaves a square and two rounded sides for the Fourth Round Square.
I found the first two using pencil and paper. The third with FreeCAD thinking in a subtractive then additive fashion. The fourth I found using OpenSCAD. The different approaches enabled finding the alternatives. I would not have found the fourth type using pencil and paper, the CAD software showed me what I described.
How would you go about finding a proof for a general formula?
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